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Category
Antibody
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Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
HIF2a
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB
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Recommended dilutions
Western blot: 0.5-1ug/ml
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Notes
The stated application concentrations are suggested starting amounts. Titration of the HIF2a antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This HIF2a antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
Q9JHS1
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Purity
Antigen affinity
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Description
HIF-2 alpha is also designated EPAS1 whose gene is mapped to 2p21-p16. The predicted mouse protein is 88% identical to human. The human gene contains 15 exons and spans at least 120 kb. The positions of the introns within the genomic region encoding the N-terminal bHLH-PAS domains of HIF2a and AHR are similar, suggesting that the 5-prime ends of the 2 genes may have arisen from a gene duplication event. Moreover, the predicted protein shares 48% sequence identity with HIF1a, a bHLH-PAS transcription factor that induces EPO gene expression in cultured cells in response to hypoxia. Like HIF1a, HIF2a binds to and activates transcription from the HIF1a response element derived from the 3-prime flanking region of the EPO gene. HIF2a is predominantly expressed in highly vascularized tissues of adult humans and in endothelial cells of the mouse adult and embryo. Furthermore, it may represent an important regulator of vascularization, perhaps involving the regulation of endothelial cell gene expression in response to hypoxia. It is expressed at relatively higher levels in villus sections of placenta and in lung samples compared with other tissues examined. In addition, the variation in HIF-2 alpha influences the relative contribution of aerobic and anaerobic metabolism and hence the maximum sustainable metabolic power for a given event duration.
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Immunogen
An amino acid sequence from the middle region of human HIF-2 alpha (HALDSENMTKSHQNLCTKGQ) was used as the immunogen for this HIF2a antibody (100% homologous in human, mouse and rat).
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Storage
After reconstitution, the HIF2a antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
