GADD45A Antibody

• Catalog number
  R30473
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  GADD45A
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the GADD45A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This GADD45A antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P24522
• Purity
  Antigen affinity
• Description
  Growth arrest and DNA-damage-inducible protein is a protein that in humans is encoded by the GADD45A gene. This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. Papathanasiou et al.(1991) demonstrated that the gene is located on 1p34-p12. Sequence analysis of human and hamster cDNA clones demonstrated that the gene has been highly conserved and encodes a novel 165-amino acid polypeptide. Northern blot analysis detected moderate expression of a 1.4-kb GADD45A transcript in heart, skeletal muscle, and kidney, with little or no expression in brain, placenta, lung, liver, and pancreas. GADD45A promotes epigenetic gene activation by repair-mediated DNA demethylation.
• Immunogen
  An amino acid sequence from the C-terminus of human GADD45A (NPHSSQWKDPALSQL) was used as the immunogen for this GADD45A antibody (100% homologous in human, mouse and rat).
• Storage
  After reconstitution, the GADD45A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  GADD45A  
• Gene symbol
  GADD45A
• Short name
  Anti-GADD45A
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to GADD45A
• Alternative technique
  antibodies
• Alternative to gene target
  growth arrest and DNA-damage-inducible, alpha, DDIT1 and GADD45, GADD45A and IDBG-99580 and ENSG00000116717 and 1647, protein binding, nuclei, Gadd45a and IDBG-153723 and ENSMUSG00000036390 and 13197, GADD45A and IDBG-638096 and ENSBTAG00000013860 and 505463

Gene info

• Identity
  HGNC:4095
• Gene
  GADD45A
• Long gene name
  growth arrest and DNA damage inducible alpha
• Synonyms gene
  • DDIT1
• Synonyms gene name
  • growth arrest and DNA-damage-inducible, alpha
• Synonyms
  • GADD45
• GenBank acession
  • M60974
• Locus
  1p31.3
• Discovery year
  1991-08-17
• Entrez gene record
  1647
• Pubmed identfication
  • 1990262
  • 8226988
  • 30617255
• RefSeq identity
  • NM_001924
• VEGA ID
  OTTHUMG00000009374

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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