EZH2 Antibody

• Catalog number
  F53360-0.4ML
• Price
  Please ask
• Size
  0.4 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  EZH2
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, ELISA
• Recommended dilutions
  Western blot: 1:2000
• Notes
  Titration of the EZH2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This EZH2 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q61188
• Purity
  Antigen affinity
• Description
  Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.
• Immunogen
  This EZH2 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 260-295 amino acids of mouse Ezh2.
• Storage
  Aliquot the EZH2 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  EZH2  
• Gene symbol
  EZH2
• Short name
  Anti-EZH2
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to EZH2
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:3527
• Gene
  EZH2
• Long gene name
  enhancer of zeste 2 polycomb repressive complex 2 subunit
• Synonyms gene name
  • enhancer of zeste (Drosophila) homolog 2
  • enhancer of zeste homolog 2 (Drosophila)
• Synonyms
  • EZH1
  • ENX-1
  • KMT6
  • KMT6A
• Locus
  7q36.1
• Discovery year
  1995-12-21
• Entrez gene record
  2146
• Pubmed identfication
  • 8954776
  • 17172412
• RefSeq identity
  • NM_004456
• Classification
  • SET domain containing
  • Polycomb repressive complex 2
  • Lysine methyltransferases
  • Myb/SANT domain containing
• VEGA ID
  OTTHUMG00000158973
• Locus Specific Databases
  • LRG_531

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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