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Category
Antibody
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Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
EphA2 Eph receptor A2
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB, IHC-P
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Recommended dilutions
Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
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Notes
The stated application concentrations are suggested starting amounts. Titration of the EphA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This EphA2 antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
P29317
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Purity
Antigen affinity
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Description
Ephrin type-A receptor 2, also known as ECK, is a protein that in humans is encoded by the EPHA2 gene. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. By screening a HeLa cell cDNA library with degenerate oligonucleotides based on highly conserved regions of receptor protein-tyrosine kinases, Lindberg and Hunter isolated cDNAs encoding EphA2, which they called ECK. The protein is readily detectable in human lens fiber cells using immunoblot and immunohistochemistry. EGFR and EphA2 mediated HCV entry by regulating CD81 -claudin-1(CLDN1) coreceptor associations and viral glycoprotein-dependent membrane fusion.
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Immunogen
An amino acid sequence from the C-terminus of human Eph receptor A2 (HQKRIAYSLLGLKDQVNTVGIPI) was used as the immunogen for this EphA2 antibody (100% homologous in human, mouse and rat).
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Storage
After reconstitution, the EphA2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Additional description
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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French translation
anticorps
