DNA polymerase beta Antibody / POLB
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Catalog number
R31898
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Price
Please ask
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Size
0.1mg
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Category
Antibody
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Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
DNA polymerase beta / POLB
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB
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Recommended dilutions
Western blot: 0.1-0.5ug/ml
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Notes
Optimal dilution of the DNA polymerase beta antibody should be determined by the researcher.
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Intented use
This DNA polymerase beta antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
P06746
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Purity
Antigen affinity
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Description
Polymerase (DNA directed), beta, also known as POLB, is an enzyme that, in humans, is encoded by the POLB gene. It is localized on 8p11.2. The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. It is found that a truncated POLB is expressed in primary colorectal tumors and inhibits the normal repair function of wildtype POLB. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. Additionally, human POLB forms a complex with and is methylated by PRMT6. In vitro, methylated POLB possesses significantly higher DNA polymerase activity when compared to that of unmodified enzyme. The increase in DNA polymerase activity upon methylation is due to the enhanced DNA binding and processivity of POLB.
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Immunogen
Amino acids 122-335 of human POLB were used as the immunogen for the DNA polymerase beta antibody.
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Storage
After reconstitution, the DNA polymerase beta antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
POLB, PRKDC, HLA-DOA
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Short name
Anti-DNA polymerase beta / POLB
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Technique
Antibody, dna, antibodies against human proteins, antibodies for
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Isotype
Rabbit IgG
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Alternative name
Antibodies to DNA polymerase beta / POLB
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Alternative technique
antibodies
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Alternative to gene target
polymerase (DNA directed), beta, POLB and IDBG-20094 and ENSG00000070501 and 102723810,5423, metal ion binding, nuclei, Polb and IDBG-140909 and ENSMUSG00000031536 and 18970, POLB and IDBG-629853 and ENSBTAG00000000225 and 614688
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Gene info
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Identity
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Gene
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Long gene name
DNA polymerase beta
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Synonyms gene name
- polymerase (DNA directed), beta
- polymerase (DNA) beta
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Locus
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Discovery year
2001-06-22
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Entrez gene record
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RefSeq identity
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Classification
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VEGA ID
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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