CrkL Antibody

• Catalog number
  R30906
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  CrkL
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the CrkL antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This CrkL antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P46109
• Purity
  Antigen affinity
• Description
  v-Crk sarcoma virus CT10 oncogene homolog(avian)-like), also called CrkL, encodes a 303-amino acid polypeptide with a predicted molecular mass of ~36 kD. While CrkL is not the human homolog of CRK, it is similar to protein-tyrosine kinases with SH2 and SH3 (src homology) domains. By fluorescence in situ hybridization,the gene is localized to chromosome 22q11, centromeric of the chronic myelogenous leukemia breakpoint region. Senechal et al.(1996)showed that CRKL becomes phosphorylated when overexpressed, activates Ras-dependent and JNK pathways, and transforms fibroblasts. The authors also found CrkL to be a substrate for the BCR-ABL tyrosine kinase, leading them to conclude that it is a tyrosine kinase and an oncogene. CrkLwas required for normal cellular responses to Fgf8, including survival and migration, Erk activation, and target gene expression.
• Immunogen
  Amino acids 286-303 (FPFTHVKIFDPQNPDENE-human) were used as the immunogen for this CrkL antibody.
• Storage
  After reconstitution, the CrkL antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  CrkL  
• Gene symbol
  CRKL
• Short name
  Anti-CrkL
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to CrkL
• Alternative technique
  antibodies
• Alternative to gene target
  v-crk sarcoma virus CT10 oncogene homolog (avian)-like, CRKL and IDBG-1833 and ENSG00000099942 and 1399, poly(A) RNA binding, Cytoplasm, Crkl and IDBG-138913 and ENSMUSG00000006134 and 12929, CRKL and IDBG-637664 and ENSBTAG00000045548 and

Gene info

• Identity
  HGNC:2363
• Gene
  CRKL
• Long gene name
  CRK like proto-oncogene, adaptor protein
• Synonyms gene name
  • v-crk avian sarcoma virus CT10 oncogene homolog-like
• Locus
  22q11.21
• Discovery year
  1994-09-14
• Entrez gene record
  1399
• Pubmed identfication
  • 8361759
  • 8798523
• RefSeq identity
  • NM_005207
• Classification
  • SH2 domain containing
• VEGA ID
  OTTHUMG00000150807

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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