CIITA Antibody
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Catalog numberR32481
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PricePlease ask
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Size0.1mg
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CategoryAntibody
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Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenCIITA
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB, IHC-P
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Recommended dilutionsWestern blot: 0.5-1ug/ml,IHC (FFPE): 1-2ug/ml
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Added bufferLyophilized from 1X Phosphate Buffered Saline (PBS) with 2.5% BSA and 0.025% sodium azide
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Intented useThis CIITA antibodyis to be used only for research purposes and not for diagnostics..
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UniprotP33076
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PurityAntigen affinity
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DescriptionCIITA is a human gene which is mapped to 16p13. This gene encodes a protein with an acidic transcriptional activation domain, 4 LRRs (leucine-rich repeats) and a GTP binding domain. The protein is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the master control factor for the expression of these genes. Also, the protein binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction. Several transcript variants encoding different isoforms have been found for this gene.
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ImmunogenAmino acids E945-R1130 from the human protein were used as the immunogen for the CIITA antibody.
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StoragePrior to reconstitution, store at 4oC. After reconstitution, the CIITA antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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LocalizationNuclear
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolCIITA
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Short nameAnti- CIITA
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameAntibodies to CIITA
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Alternative techniqueantibodies
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Alternative to gene targetclass II, major histocompatibility complex, transactivator, C2TA and CIITAIV and MHC2TA and NLRA, CIITA and IDBG-14377 and ENSG00000179583 and 4261, transferase activity, nuclei, Ciita and IDBG-132343 and ENSMUSG00000022504 and 12265, CIITA and IDBG-636856 and ENSBTAG00000020649 and
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Gene info
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Identity
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Gene
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Long gene nameclass II major histocompatibility complex transactivator
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Synonyms gene
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Synonyms gene name
- MHC class II transactivator
- class II, major histocompatibility complex, transactivator
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-06-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- NLR family
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data