CDC42 Antibody

• Catalog number
  R30435
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  CDC42
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Bovin, Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the CDC42 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This CDC42 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P60953
• Purity
  Antigen affinity
• Description
  Cell division control protein 42 homolog also known as CDC42 is a protein involved in regulation of the cell cycle. In humans, CDC42 is encoded by the CDC42 gene. CDC42 is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein(N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.
• Immunogen
  An amino acid sequence from the middle region of human CDC42 (DDPSTIEKLAKNKQKPIT) was used as the immunogen for this CDC42 antibody (100% homologous in human, mouse and rat).
• Storage
  After reconstitution, the CDC42 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  CDC42  
• Gene symbol
  CDC42-AS1, CDC42-IT1, CDC42
• Short name
  Anti-CDC42
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to CDC42
• Alternative technique
  antibodies
• Alternative to gene target
  cell division cycle 42 (GTP binding protein, 25kDa), CDC42Hs and G25K, CDC42 and IDBG-93614 and ENSG00000070831 and 998, identical protein binding, Cell surfaces, Cdc42 and IDBG-198471 and ENSMUSG00000006699 and 12540, BT.56113 and IDBG-645532 and ENSBTAG00000001700 and 532712

Gene info

• Identity
  HGNC:54314
• Gene
  CDC42-AS1
• Long gene name
  CDC42 antisense RNA 1
• Locus
  1p36.12
• Discovery year
  2019-05-03
• Entrez gene record
  112268223
• Classification
  • Antisense RNAs

Gene info

• Identity
  HGNC:41317
• Gene
  CDC42-IT1
• Long gene name
  CDC42 intronic transcript 1
• Synonyms gene name
  • CDC42 intronic transcript 1 (non-protein coding)
• Locus
  1p36.12
• Discovery year
  2011-05-19
• Classification
  • Intronic transcripts
• VEGA ID
  OTTHUMG00000002890

Gene info

• Identity
  HGNC:1736
• Gene
  CDC42
• Long gene name
  cell division cycle 42
• Synonyms gene name
  • cell division cycle 42 (GTP-binding protein, 25kD)
  • cell division cycle 42 (GTP binding protein, 25kDa)
• Synonyms
  • G25K
  • CDC42Hs
• Synonyms name
  • GTP binding protein, 25kDa
• GenBank acession
  • BC018266
• Locus
  1p36.12
• Discovery year
  1991-06-06
• Entrez gene record
  998
• Pubmed identfication
  • 2124704
  • 2122236
• RefSeq identity
  • NM_001791
• Classification
  • Rho family GTPases
• VEGA ID
  OTTHUMG00000002753
• Locus Specific Databases
  • LRG_1326

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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