CCR10 Antibody

• Catalog number
  R30564
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  CCR10
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the CCR10 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This CCR10 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P46092
• Purity
  Antigen affinity
• Description
  C-C chemokine receptor type 10 is a protein that in humans is encoded by the CCR10 gene. It is constitutively expressed in skin melanocytes, fibroblasts, and microvascular endothelial cells, and that it is upregulated by IL1B and TNF. Expression was also detected in T cells, Langerhans cells, and peripheral blood mononuclear cells but not in dendritic cells. By fluorescence in situ hybridization, the gene is mapped to 17q21.1-q21.3. CCR10 is the receptor for CCL27. CCR10-CCL27 interactions are involved in T cell-mediated skin inflammation. expression of CCL27 in normal keratinocytes and its strong upregulation in skin lesions of atopic dermatitis, contact dermatitis, and psoriasis patients. CCR10+ T lymphocytes were detected in lesional but not normal skin of these patients. Flow cytometric analysis showed that CCL27 binds extracellular matrix components and dermal microvascular endothelial cells and fibroblasts and mediates adhesion and transendothelial migration of CCR10+ circulating leukocytes. The protein is predominantly expressed on CD4+CLA+(cutaneous lymphocyte antigen), rather than CD8+, circulating T cells. RT-PCR, confocal microscopy, and ELISA analysis indicated that keratinocytes exposed to TNF or IL1B but not to IL4 or IFNG in vitro express increased CCL27.
• Immunogen
  Amino acids 312-327 (FLGLRFRQDLRRLLRG-human) were used as the immunogen for this CCR10 antibody.
• Storage
  After reconstitution, the CCR10 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  CCR10  
• Gene symbol
  CCR10, ACKR2
• Short name
  Anti-CCR10
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to CCR10
• Alternative technique
  antibodies
• Alternative to gene target
  chemokine (C-C motif) receptor 10, GPR2, CCR10 and IDBG-51414 and ENSG00000184451 and 2826, C-C chemokine receptor activity, Plasma membranes, Ccr10 and IDBG-211910 and ENSMUSG00000044052 and 12777, CCR10 and IDBG-640334 and ENSBTAG00000019095 and 539108

Gene info

• Identity
  HGNC:4474
• Gene
  CCR10
• Long gene name
  C-C motif chemokine receptor 10
• Synonyms gene
  • GPR2
• Synonyms gene name
  • G protein-coupled receptor 2
  • chemokine (C-C motif) receptor 10
• GenBank acession
  • AF215981
• Locus
  17q21.2
• Discovery year
  1994-07-25
• Entrez gene record
  2826
• Pubmed identfication
  • 7851889
• RefSeq identity
  • NM_016602
• Classification
  • C-C motif chemokine receptors
• VEGA ID
  OTTHUMG00000132301

Gene info

• Identity
  HGNC:1565
• Gene
  ACKR2
• Long gene name
  atypical chemokine receptor 2
• Synonyms gene
  • CMKBR9
  • CCBP2
• Synonyms gene name
  • chemokine binding protein 2
• Synonyms
  • CCR10
  • D6
  • CCR9
• GenBank acession
  • U94888
• Locus
  3p22.1
• Discovery year
  1998-04-28
• Entrez gene record
  1238
• Pubmed identfication
  • 9364936
  • 9405404
  • 16148
• RefSeq identity
  • NM_001296
• Classification
  • Atypical chemokine receptors
• VEGA ID
  OTTHUMG00000186377

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products