BSP Antibody Bone Sialoprotein

• Catalog number
  R30987
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  BSP Bone Sialoprotein
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the BSP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This BSP antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q61711
• Purity
  Antigen affinity
• Description
  Integrin-binding sialoprotein, also known as Bone sialoprotein and BSP, is a major structural protein of the bone matrix. Bone sialoprotein is a predicted 35KD acidic glycoprotein that undergoes extensive posttranslational modifications and is visualized by western blot at up to 70KD. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. The BSP gene is mapped to 4q22.1.
• Immunogen
  An amino acid sequence from the C-terminus of mouse Bone Sialoprotein (SYYKGHGYEGYEGQNYYYHQ) was used as the immunogen for this BSP antibody.
• Storage
  After reconstitution, the BSP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  BSP   Bone   Sialoprotein  
• Gene symbol
  IBSP
• Short name
  Anti-BSP Bone Sialoprotein
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to BSP Bone Sialoprotein
• Alternative technique
  antibodies
• Tissue
  bone

Gene info

• Identity
  HGNC:5341
• Gene
  IBSP
• Long gene name
  integrin binding sialoprotein
• Synonyms
  • BSP
  • SP-II
  • BSP-II
• Synonyms name
  • bone sialoprotein
  • bone sialoprotein II
• Locus
  4q22.1
• Discovery year
  1993-03-23
• Entrez gene record
  3381
• Pubmed identfication
  • 8406493
• Classification
  • SIBLING family
• VEGA ID
  OTTHUMG00000130600

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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