BiP Antibody GRP78
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Catalog numberR30913
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PricePlease ask
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Size0.1mg
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CategoryAntibody
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Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenBiP GRP78
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB, IHC-P, IHC-F, ICC
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Recommended dilutionsWestern blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
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NotesThe stated application concentrations are suggested starting points. Titration of the BiP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis BiP antibodyis to be used only for research purposes and not for diagnostics..
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UniprotP11021
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PurityAntigen affinity
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DescriptionHSPA5 (heat shock 70kDa protein 5) also known as glucose-regulated protein, 78kD(GRP78) or BiP, is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum.BiP is an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by theproteasome. Shen et al.(2002)concluded thatBiPretains ATF6 in the ER by inhibiting its Golgi localization signals and that dissociation ofBiPduring ER stress allows ATF6 to be transported to the Golgi. The findings ofShen et al.(2002)demonstrated thatthe proteinis a key element in sensing the folding capacity within the ER.
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ImmunogenAn amino acid sequence from the C-terminus of human GRP78/BiP (EWLESHQDADIEDFK) was used as the immunogen for this BiP antibody (100% homologous in human, mouse and rat).
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StorageAfter reconstitution, the BiP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolHSPA5
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Short nameAnti-BiP GRP78
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit Ig
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Alternative nameAntibodies to BiP GRP78
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameheat shock protein family A (Hsp70) member 5
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Synonyms gene
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Synonyms gene name
- heat shock 70kD protein 5 (glucose-regulated protein, 78kD)
- heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
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Synonyms
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Synonyms name
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Locus
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Discovery year1991-07-26
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Entrez gene record
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Classification
- Heat shock 70kDa proteins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data