Beclin 1 Antibody
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Catalog numberR30945
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PricePlease ask
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Size0.1mg
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CategoryAntibody
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Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenBeclin 1
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB
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Recommended dilutionsWestern blot: 0.5-1ug/ml
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NotesTitration of the Beclin 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis Beclin 1 antibodyis to be used only for research purposes and not for diagnostics..
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UniprotQ14457
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PurityAntigen affinity
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DescriptionBellini-1 is a protein that in humans is encoded by the BECN1 gene, also known as BECN1, ATG6, VPS30, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, autophagy-related gene 6 (ATG6). Beclin 1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagosome in autophagy. The gene encodes a novel 450-amino acid protein containing a coiled-coil region, within which it has limited homology to myosin-like proteins. Northern blot analysis of mouse and adult human tissues revealed widespread expression. A 2.2-kb transcript was present at highest levels in human skeletal muscle and at detectable levels in all tissues examined. In some tissues, additional 1.7- and 1.4-kb transcripts were observed, suggesting the presence of alternatively spliced transcripts. Immunoperoxidase staining of human hippocampus and frontal cortex sections revealed Beclin 1 immunoreactivity in many neurons throughout these regions, as well as in some glial cells. It protects against infection by a neurovirulent strain of Sindbis virus that is known to overcome the protective effects of Bcl2. Liang et al. showed that Beclin 1 reduced Sindbis virus replication in mouse brain and reduced Sindbis virus-induced cell death in mouse brain.
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ImmunogenAmino acids 222-237 (LDQEEAQYQREYSEFK) were used as the immunogen for this Beclin 1 antibody (100% homologous in human, mouse and rat).
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StorageStore the lyophilized Beclin 1 antibody at 4oC. After reconstitution, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolBECN2, BECN1, BECN1P2
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Short nameAnti-Beclin 1
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameAntibodies to Beclin 1
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namebeclin 2
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Synonyms gene
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Synonyms gene name
- beclin 1, autophagy related, pseudogene 1
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Locus
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Discovery year2010-06-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namebeclin 1
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Synonyms gene name
- beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)
- beclin 1, autophagy related
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-11-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- PIK3C3 complex subunits
- MicroRNA protein coding host genes
- Autophagy related
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namebeclin 1 pseudogene 2
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Synonyms gene name
- beclin 1, autophagy related, pseudogene 2
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Locus
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Discovery year2014-10-03
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Entrez gene record
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data