Bad Antibody
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Catalog numberF42825-0.4ML
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PricePlease ask
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Size0.4 ml
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CategoryAntibody
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ConcentrationIn 1X PBS, pH 7.4, with 0.09% sodium azide
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FormPurified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenBad
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens), Mouse (Mus musculus) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB, IHC, FACS, ELISA
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Recommended dilutionsWestern blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
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NotesTitration of the Bad antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis Bad antibodyis to be used only for research purposes and not for diagnostics..
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UniprotQ92934
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PurityPurified
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DescriptionApoptosis or programmed cell death is a physiological cellular process characterized by cell shrinkage, membrane blebbing, DNA fragmentation, and release of Cytochrome C from the mitochondria. It is utilized by the organism to get rid of unwanted cells, which is critical for normal development and homeostasis of an organism. Disregulation of normal apoptosis process have been implicated in a variety of diseases, including cancer, autoimmune diseases, viral infections, etc. Programmed cell death occurs through complex cascades of cell signaling in which Bcl-2 family members, among others, play an important role.The Bcl-2 family of proteins regulate apoptosis as well as execute death signals at the mitochondrion. Members of this family include both pro- and anti-apoptotic proteins that hare homology sequences called Bcl-2 Homology domains (BH1-4) which mediate dimmer formation. The BH3 proteins, such as BID, NOXA, PUMA, BIK, BIM and BAD are all pro-apoptotic and share sequence homology within the amphipathic alpha-helical BH3 region, which is required for their apoptotic function. They may trigger release of death-inducing molecules such as Cytochrome C, Smac, and endonuclease G. Anti-apoptotic family members, including Bcl-2 and Bcl-XL, play inhibitory roles. Bcl-2 family proteins may form homodimers or heterodimers between pro- and anti-apoptotic members, the ratios of which determine the cell fate.
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ImmunogenA portion of amino acids 92-127 from the human protein was used as the immunogen for this Bad antibody.
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StorageAliquot the Bad antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolBAD, LAMP5, SNRNP200
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Short nameAnti-Bad
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit Ig
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Alternative nameAntibodies to Bad
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Alternative techniqueantibodies
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Alternative to gene targetBCL2-associated a this GO nist of cell death, BBC2 and BCL2L8, BAD and IDBG-53423 and ENSG00000002330 and 572, protein heterodimerization activity, Cytoplasm, Bad and IDBG-137335 and ENSMUSG00000024959 and 12015, BT.55802 and IDBG-643918 and ENSBTAG00000012511 and 615013
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Gene info
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Identity
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Gene
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Long gene nameBCL2 associated agonist of cell death
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-10-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- BCL2 homology region 3 (BH3) only
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namelysosomal associated membrane protein family member 5
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Synonyms gene
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Synonyms gene name
- chromosome 20 open reading frame 103
- lysosomal-associated membrane protein family, member 5
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-07-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Lysosome associated membrane proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nuclear ribonucleoprotein U5 subunit 200
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Synonyms gene
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Synonyms gene name
- activating signal cointegrator 1 complex subunit 3-like 1
- retinitis pigmentosa 33 (autosomal dominant)
- small nuclear ribonucleoprotein 200kDa (U5)
- small nuclear ribonucleoprotein, U5 200kDa subunit
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-08-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Spliceosomal C complex
- U5 small nuclear ribonucleoprotein
- RNA helicases
- Spliceosomal Bact complex
- Spliceosomal P complex
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
