ATG5 Antibody

• Catalog number
  R31384
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  ATG5
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting points. Titration of the ATG5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This ATG5 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q9H1Y0
• Purity
  Antigen affinity
• Description
  Autophagy protein 5 is a protein that in humans is encoded by the ATG5 gene. It is also known as APG5 or ASP, and this gene is mapped to 6q21. It is found that knockdown of the protein in hepatocytes increased triglyceride levels with oleate or a second endogenous stimulus for triglyceride formation. These knockdown hepatocytes also had increased lipid droplet number and size. ATG5 is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II.
• Immunogen
  Amino acids 82-97 (DRFDQFWAINRKLMEY-human) were used as the immunogen for this ATG5 antibody (100% mouse homology).
• Storage
  After reconstitution, the ATG5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  ATG5  
• Gene symbol
  ATG5
• Short name
  Anti-ATG5
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to ATG5
• Alternative technique
  antibodies
• Alternative to gene target
  autophagy related 5, APG5 and APG5-LIKE and APG5L and ASP and hAPG5, ATG5 and IDBG-94856 and ENSG00000057663 and 9474, protein binding, Plasma membranes, Atg5 and IDBG-148568 and ENSMUSG00000038160 and 11793, ATG5 and IDBG-631895 and ENSBTAG00000005400 and 532686

Gene info

• Identity
  HGNC:589
• Gene
  ATG5
• Long gene name
  autophagy related 5
• Synonyms gene
  • APG5L
• Synonyms gene name
  • APG5 (autophagy 5, S. cerevisiae)-like
  • APG5 autophagy 5-like (S. cerevisiae)
  • ATG5 autophagy related 5 homolog (S. cerevisiae)
• Synonyms
  • ASP
  • APG5
  • hAPG5
• GenBank acession
  • Y11588
• Locus
  6q21
• Discovery year
  1999-09-16
• Entrez gene record
  9474
• Pubmed identfication
  • 9563500
  • 11349150
  • 15778222
• RefSeq identity
  • NM_004849
• Classification
  • Autophagy related
• VEGA ID
  OTTHUMG00000016193

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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