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Category
Antibody
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Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
Arg2
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB, IHC-P
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Recommended dilutions
Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
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Notes
The stated application concentrations are suggested starting amounts. Titration of the Arg2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This Arg2 antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
P78540
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Purity
Antigen affinity
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Description
Arginase, type II encodes a 355-amino acid polypeptide. Using Northern blotting and RT-PCR, Vockley et al.(1996) found that ARG2 is expressed as a 1.5-kb mRNA in a wide variety of tissues, with highest levels of expression in prostate, brain, and kidney. ARG2 may be inducible and may be essential in the regulation of nitric oxide synthesis by modulating local arginine concentrations. Gotoh et al.(1996) showed that ARG2 mRNA and nitric oxide synthase (NOS) mRNA were coinduced by lipopolysaccharide in a macrophage-like cell line. Arginase II has been implicated in the regulation of the arginine/ornithine concentrations in the cell. The mitochondrial location of the protein and its coinduction with ornithine aminotransferase and involvement with proline biosynthesis in lactating rat mammary gland had led to the inference that it is involved in biosynthetic functions, as opposed to the metabolic ones of the urea cycle.
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Immunogen
An amino acid sequence from the N-terminus of human Arginase II (DFGDLSFTPVPKDDLYNNL) was used as the immunogen for this Arg2 antibody.
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Storage
After reconstitution, the Arg2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
