APOB Antibody

• Catalog number
  R32510
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  APOB
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.5-2ug/ml,IHC (FFPE): 1-2ug/ml
• Added buffer
  Lyophilized from 1X Phosphate Buffered Saline (PBS) with 2.5% BSA and 0.025% sodium azide
• Intented use
  This APOB antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P04114
• Purity
  Antigen affinity
• Description
  Apolipoprotein B (ApoB) is a protein that in humans is encoded by the APOB gene. This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.
• Immunogen
  Amino acids Q246-N450 from the human protein were used as the immunogen for the APOB antibody. This sequence is common to both the full length (ApoB-100) and truncated (ApoB-48) forms of the protein.
• Storage
  After reconstitution, the APOB antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Localization
  Cytoplasmic, membranous
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  APOB  
• Gene symbol
  APOB
• Short name
  Anti- APOB
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to APOB
• Alternative technique
  antibodies
• Alternative to gene target
  apolipoprotein B (including Ag(x) antigen), FLDB and LDLCQ4, APOB and IDBG-32746 and ENSG00000084674 and 338, low-density lipoprotein particle receptor binding, Extracellular, Apob and IDBG-128209 and ENSMUSG00000020609 and 238055, APOB and IDBG-637535 and ENSBTAG00000008505 and 494004

Gene info

• Identity
  HGNC:603
• Gene
  APOB
• Long gene name
  apolipoprotein B
• Synonyms gene name
  • apolipoprotein B (including Ag(x) antigen)
• GenBank acession
  • M14162
• Locus
  2p24.1
• Discovery year
  2001-06-22
• Entrez gene record
  338
• Classification
  • Apolipoproteins
• VEGA ID
  OTTHUMG00000090785

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products