APAF1 Antibody

• Catalog number
  R30355
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  APAF1
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the APAF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This APAF1 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  O14727
• Purity
  Antigen affinity
• Description
  Apoptotic peptidase activating factor 1, also known as APAF1, is a protein which in humans is encoded by the APAF1 gene. This gene is mapped to chromosome 12q23. It encodes a cytoplasmic protein that initiates apoptosis. And it is an essential downstream effector of p53-mediated apoptosis. This protein contains several copies of the WD40 repeat domain, a caspase recruitment domain(CARD), and an ATPase domain(NB-ARC). In the presence of cytochrome c and dATP, APAF1 assembles into an oligomeric apoptosome, which is responsible for activation of procaspase-9 and maintenance of the enzymatic activity of processed caspase-9. Furthermore, APAF1 is inactivated in metastatic melanomas, leading to defects in the execution of apoptotic cell death. Additionally, APAF1 has been shown to interact with NLRP1, Caspase-9, APIP, BCL2-like 1 and HSPA4.
• Immunogen
  An amino acid sequence from the N-terminus of human APAF1 (MDAKARNCLLQHREALEKD) was used as the immunogen for this APAF1 antibody (100% homologous in human and mouse).
• Storage
  After reconstitution, the APAF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  APAF1  
• Gene symbol
  APAF1
• Short name
  Anti-APAF1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to APAF1
• Alternative technique
  antibodies
• Alternative to gene target
  apoptotic peptidase activating factor 1, APAF-1 and CED4, APAF1 and IDBG-52364 and ENSG00000120868 and 317, ADP binding, nuclei, Apaf1 and IDBG-185032 and ENSMUSG00000019979 and 11783, APAF1 and IDBG-636787 and ENSBTAG00000021661 and 537782

Gene info

• Identity
  HGNC:576
• Gene
  APAF1
• Long gene name
  apoptotic peptidase activating factor 1
• Synonyms gene name
  • apoptotic protease activating factor
  • apoptotic peptidase activating factor
• Synonyms
  • CED4
  • APAF-1
• GenBank acession
  • AF013263
• Locus
  12q23.1
• Discovery year
  1998-01-20
• Entrez gene record
  317
• Pubmed identfication
  • 9267021
  • 10702682
• RefSeq identity
  • NM_181861.1
• Classification
  • Apoptosome
  • WD repeat domain containing
  • Caspase recruitment domain containing
• VEGA ID
  OTTHUMG00000170214

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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