ADAMTS5 Antibody

• Catalog number
  R31183
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  ADAMTS5
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the ADAMTS5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This ADAMTS5 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q9UNA0
• Purity
  Antigen affinity
• Description
  A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 5, is an enzyme that in humans is encoded by the ADAMTS5 gene. It is a member of the large ADAMTS family of zinc-dependent proteases. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage. By somatic cell hybrid analysis, Hurskainen et al.(1999) mapped the human ADAMTS5 gene to chromosome 21. Using mouse models, Echtermeyer et al.(2009) showed that Sdc4 controls a pathway that activates Adamts5 at the chondrocyte cell surface through Erk1/Erk2 activation of Mmp3.
• Immunogen
  An amino acid sequence from the C-terminus of human ADAMTS5 (TAYLALKKKNGEYLIN) was used as the immunogen for this ADAMTS5 antibody.
• Storage
  After reconstitution, the ADAMTS5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  ADAMTS5  
• Gene symbol
  ADAMTS5
• Short name
  Anti-ADAMTS5
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to ADAMTS5
• Alternative technique
  antibodies
• Alternative to gene target
  ADAM metallopeptidase with thrombospondin type 1 motif, 5, ADAM-TS 11 and ADAM-TS 5 and ADAM-TS5 and ADAMTS-11 and ADAMTS-5 and ADAMTS11 and ADMP-2, ADAMTS5 and IDBG-1207 and ENSG00000154736 and 11096, zinc ion binding, Extracellular, Adamts5 and IDBG-173252 and ENSMUSG00000022894 and 23794, ADAMTS5 and IDBG-630284 and ENSBTAG00000000648 and 286805

Gene info

• Identity
  HGNC:221
• Gene
  ADAMTS5
• Long gene name
  ADAM metallopeptidase with thrombospondin type 1 motif 5
• Synonyms gene name
  • a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2)
• Synonyms
  • ADMP-2
  • ADAMTS11
• Synonyms name
  • aggrecanase-2
• GenBank acession
  • AF142099
• Locus
  21q21.3
• Discovery year
  1999-08-23
• Entrez gene record
  11096
• Pubmed identfication
  • 10438522
• Classification
  • ADAM metallopeptidases with thrombospondin type 1 motif
• VEGA ID
  OTTHUMG00000078686

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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