Transferrin Receptor 1, Human, Western Blot Positive Control (TfR1)

• Catalog number
  MBS652634
• Price
  Please ask
• Size
  5x0.1 mL

• Products_type
  Immunoglobulin
• Products_short_name
  [Transferrin Receptor 1, Western Blot Positive Control]
• Reactivity
  Human
• Purity
  Highly Purified Purified using Protein A/G column chromatography (~95%).
• Form
  Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
• Storage_stability
  Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Tested_application
  Western Blot (WB)
• Test
  Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.
• Description
  Isotype or positive controls by peptides, antibodies and deactivated samples. Positive controls are the same as the target vector or antibody or protein and can be spiked to the sample before the analysis starts. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Group
  positif

• Gene target
  Transferrin   Receptor   TfR1  
• Short name
  Transferrin Receptor 1, , Blot Positive Control (TfR1)
• Technique
  Western Blot, Positive, Control, Blot, western Blots, controls
• Host
  Human placenta and blood
• Species
  Human, Humans
• Alternative name
  Transferrin Receptor 1, H. sapiens, Western Blot Positive reference (TfR1)
• Alternative technique
  controls, western
• Tissue
  control

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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