TRA1-60 (TRA-1, Tumor Rejection Antigen 1)

Products_type

Antibody
Products_short_name

[TRA1-60]
Products_name_syn

[Anti -TRA1-60 (TRA-1, Tumor Rejection Antigen 1)]
Clonality

Monoclonal
Clone

[5C2]
Reactivity

Human
Specificity

Recognizes TRA-1-60 antigen that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Recognizes antigens that are associated with a pericellular matrix proteoglycan. Reacts with a sialidase-sensitive epitope.
Purity

Purified Purified
Form

Supplied as a liquid in PBS, pH 8.0, 0.05% sodium azide.
Storage_stability

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Tested_application

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Description

Antigens are peptides or recombinant or native dependent on the production method.

Gene target

TRA1-60 TRA-1 Tumor Rejection
Gene symbol

HSP90B1, TRRAP, IGHVII-60-1, TRA, IGLV4-60, TRA-TGC3-1, IGHV3-60, TRA-TGC1-1, TRA-CGC1-1, TRA-AGC1-1
Short name

TRA1-60 (TRA-1, Tumor Rejection Antigen 1)
Technique

antigen, antigenes
Host

Mouse
Isotype

IgM
Alternative name

TRA1-60 (TRA-1, Tumor Rejection protein 1)
Alternative technique

antigenes
Tissue

tumor

Identity

HGNC:12028
Gene

HSP90B1
Long gene name

heat shock protein 90 beta family member 1
Synonyms gene
- TRA1
Synonyms gene name
- tumor rejection antigen (gp96) 1
- heat shock protein 90kDa beta (Grp94), member 1
Synonyms
- GP96
- GRP94
Synonyms name
- endoplasmin
GenBank acession
- AY040226
Locus

12q23.3
Discovery year

1991-07-03
Entrez gene record

7184
Pubmed identfication
- 16269234
RefSeq identity
- NM_003299
Classification
- Heat shock 90kDa proteins
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000170118

Identity

HGNC:12347
Gene

TRRAP
Long gene name

transformation/transcription domain associated protein
Synonyms gene name
- transformation/transcription domain-associated protein
Synonyms
- TR-AP
- PAF400
- Tra1
GenBank acession
- AF076974
Locus

7q22.1
Discovery year

1998-10-06
Entrez gene record

8295
Pubmed identfication
- 9708738
- 9885574
RefSeq identity
- NM_003496
Classification
- MicroRNA protein coding host genes
- SAGA complex
- Armadillo like helical domain containing
VEGA ID

OTTHUMG00000150403

Identity

HGNC:5686
Gene

IGHVII-60-1
Long gene name

immunoglobulin heavy variable (II)-60-1 (pseudogene)
Synonyms gene name
- immunoglobulin heavy variable (II)-60-1
- immunoglobulin heavy variable (II)-60-1 pseudogene
Synonyms
- IGHV(II)-60-1
GenBank acession
- AB019437
Locus

14q32.33
Discovery year

2000-04-17
Entrez gene record

28361
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000151951

Identity

HGNC:34846
Gene

TRA-TGC3-1
Long gene name

tRNA-Ala (anticodon TGC) 3-1
Synonyms gene
- TRNAA26
- TRA-TGC3-2
Synonyms gene name
- transfer RNA alanine 26 (anticodon UGC)
- transfer RNA-Ala (TGC) 3-2
- transfer RNA-Ala (TGC) 3-1
Synonyms
- tRNA-Ala-TGC-3-1
GenBank acession
- HG983586
Locus

5q35.3
Discovery year

2008-08-29
Entrez gene record

100189280
Classification
- Cytoplasmic transfer RNAs

Identity

HGNC:5614
Gene

IGHV3-60
Long gene name

immunoglobulin heavy variable 3-60 (pseudogene)
Synonyms gene name
- immunoglobulin heavy variable 3-60
- immunoglobulin heavy variable 3-60 pseudogene
GenBank acession
- M29813
Locus

14q32.33
Discovery year

2000-04-04
Entrez gene record

28417
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000151956

Identity

HGNC:34711
Gene

TRA-TGC1-1
Long gene name

tRNA-Ala (anticodon TGC) 1-1
Synonyms gene
- TRNAA19
Synonyms gene name
- transfer RNA alanine 19 (anticodon UGC)
- transfer RNA-Ala (TGC) 1-1
Synonyms
- tRNA-Ala-TGC-1-1
GenBank acession
- HG983583
Locus

6p22.1
Discovery year

2008-08-29
Entrez gene record

100189149
Classification
- Cytoplasmic transfer RNAs

Identity

HGNC:34875
Gene

TRA-CGC1-1
Long gene name

tRNA-Ala (anticodon CGC) 1-1
Synonyms gene
- TRNAA29
Synonyms gene name
- transfer RNA alanine 29 (anticodon CGC)
- transfer RNA-Ala (CGC) 1-1
Synonyms
- tRNA-Ala-CGC-1-1
GenBank acession
- HG983578
Locus

6p22.2
Discovery year

2008-08-29
Entrez gene record

100189308
Classification
- Cytoplasmic transfer RNAs

Identity

HGNC:34914
Gene

TRA-AGC1-1
Long gene name

tRNA-Ala (anticodon AGC) 1-1
Synonyms gene
- TRNAA34
Synonyms gene name
- transfer RNA alanine 34 (anticodon AGC)
- transfer RNA-Ala (AGC) 1-1
Synonyms
- tRNA-Ala-AGC-1-1
GenBank acession
- HG983548
Locus

6p22.1
Discovery year

2008-08-29
Entrez gene record

100189346
Classification
- Cytoplasmic transfer RNAs

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data