-
Products_type
Antibody
-
Products_short_name
[TNF-alpha (Tumor Necrosis Factor alpha)]
-
Products_name_syn
[APC1, Cachectin, Differentiation inducing factor (DIF), Macrophage cytotoxic factor (MCF), Necrosin, TNF alpha, TNF Macrophage Derived, TNF Monocyte Derived, TNF Superfamily Member 2, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor Precursor]
-
Other_names
[tumor necrosis factor; Tumor necrosis factor; tumor necrosis factor; tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a]
-
Products_gene_name
[TNF]
-
Other_gene_names
[TNF; TNF; DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha; TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2]
-
Clonality
Monoclonal
-
Clone
[TNF706 + P/T2]
-
Reactivity
Human, Mouse, Rat, Rabbit, Cat, Dog, Zebrafish. Others not known.
-
Specificity
TNF alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha causes cytolysis of certain transformed cells. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
-
Form
200ug/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
-
Storage_stability
Antibody with azide - store at 2 to 8°C.
-
Tested_application
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin/Paraffin
-
Description
The TNF-alpha (Tumor Necrosis Factor alpha) Mouse Monoclonal Antibody is a α- or alpha protein sometimes glycoprotein present in blood. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
-
Properties
If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
Gene
Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.
-
French translation
anticorps
-
Gene target
-
Gene symbol
TNF, TNFRSF1A, TNFRSF1B, LTBR
-
Short name
TNF-alpha (Tumor Necrosis Factor alpha) Mouse Monoclonal Antibody
-
Technique
Mouse monoclonal, Antibody, Mouse, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
-
Host
Mouse, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
-
Isotype
Mouse/IgM, kappa( TNF706)+ Mouse/IgM kappa (P/T2)
-
Species
Mouse, Mouses
-
Alternative name
tumor necrosis factor-a (Tumor Necrosis Factor a) Mouse monoclonal (antibody to-)
-
Alternative technique
antibodies, murine
-
Alternative to gene target
tumor necrosis factor, DIF and TNF-alpha and TNFA and TNFSF2, TNF and IDBG-300259 and ENSG00000232810 and 7124, transcription regulatory region DNA binding, Extracellular, Tnf and IDBG-177358 and ENSMUSG00000024401 and 21926, TNF and IDBG-634161 and ENSBTAG00000025471 and 280943
-
Tissue
tumor
-
