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Products_type
Recombinant Protein
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Products_short_name
[Tissue Inhibitor of Metalloproteinases 1, Western Blot Control]
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Purity
PurifiedPurified
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Form
Supplied as a lyophilized powder from conditioned media.
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Storage_stability
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested_application
ELISA (EL/EIA), Western Blot (WB), Gel Shift Assay (GS/EMSA)
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Test
Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples. Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme. 6
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Latin name
Mus musculus
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
TIMP1
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Short name
Tissue Inhibitor of Metalloproteinases 1, Recombinant, Mouse, Blot Control (TIMP1, Erythroid Potentiating Activity, EPA, Collagenase Inhibitor, CI)
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Technique
Western Blot, Recombinant, Control, tissue, Mouse, Blot, western Blots, controls, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture. tissues
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Host
mouse
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Species
Mouse, Mouses
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Alternative name
Tissue suppressor on Metalloproteinases 1, Rec., Mouse, Western Blot reference (TIMP1, Erythroid Potentiating Activity, EPA, Collagenase suppressor, CI)
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Alternative technique
rec, controls, tissues, murine, western
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Tissue
control, tissue
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