Texas Red Conjugated Affinity Purified anti-Rat IgG [H&L] [Donkey]
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Catalog number
MBS418008
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Price
Please ask
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Size
0.25 mg
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Products_type
Secondary Antibody
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Products_short_name
[IgG [H&L]]
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Products_gene_name
[IgG]
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Reactivity
Rat
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Concentration
1.0 mg/ml (by UV absorbance at 280 nm)
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Storage_stability
For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
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Tested_application
Immunomicroscopy, Flow Cytometry (FC/FACS), Other antibody based fluorescent assays requiring lot-to-lot consistency.
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Test
A high affinity purification column was use to purify Red Conjugated anti- IgG [H L] [ ] by MyBioSource by chromatographic size exclusion.
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Description
1 of protein A or G purified by MyBioSource will give more specificity than crude serum. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Latin name
Equus africanus asinus, Rattus norvegicus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Conjugation
red dye
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Gene target
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Short name
Red Conjugated anti- IgG [H L] [ ]
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Technique
affinity, purified, anti-, anti, IgG, antibody to, antibodies, antibody Conjugates, IgGs, purifications
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Host
Donkey, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
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Isotype
IgG, Heavy & Light chains, IgG
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Label
Texas
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Species
Rat, Donkeys, Rats
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Alternative name
Texas Red coupled protein enriched Antibody toRat Immunoglobulin G [heavy + light chain] [Donkey]
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Alternative technique
pure, antibodies, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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