TCR, Cbeta1 (T Cell Receptor) (FITC)

• Catalog number
  MBS604273
• Price
  Please ask
• Size
  5x120 Tests

• Products_type
  Antibody
• Products_short_name
  [TCR, Cbeta1]
• Products_name_syn
  [Anti -TCR, Cbeta1 (T Cell Receptor) (FITC)]
• Other_names
  [T cell receptor beta chain]
• Clonality
  Monoclonal
• Clone
  [7H199]
• Reactivity
  Human
• Specificity
  Recognizes human C beta 1 TCR gene product. Reacts with 50-75% of T cells in normal human blood. Is a mitogenic for T cells expressing TCR C beta 1.
• Purity
  Affinity Purified Purified by Protein A affinity chromatography.
• Form
  Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with fluorescein isothiocyanate (FITC).
• Storage_stability
  May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
• Tested_application
  Flow Cytometry (FC/FACS)
• Description
  For cells, cell lines and tissues in culture till half confluency. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody

• Gene target
  TCR   Cbeta1   Receptor  
• Short name
  TCR, Cbeta1 (T Receptor) (FITC)
• Technique
  FITC, Fluorescein
• Host
  Mouse
• Isotype
  IgG2a
• Label
  FITC
• Alternative name
  TCR, Cbeta1 (T cellular Receptor) (fluorecein)
• Alternative technique
  fluorescine
• Tissue
  cell

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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