Swine anti Human IgG, heavy and light chains, conjugated with Biotin

• Catalog number
  MBS571875
• Price
  Please ask
• Size
  5x1 mL

• Products_type
  Secondary Antibody
• Products_short_name
  [Swine anti Human IgG, heavy and light chains]
• Clonality
  Polyclonal
• Reactivity
  Human
• Specificity
  Biotin-conjugated IgG fraction of polyclonal Swine antiSerum to Human IgG, heavy and light chains.
• Storage_stability
  Store at 4 degree C.
• Tested_application
  ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), Dot Blot (DB), Immunoblotting (IB)
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Conjugation
  Biotinylated
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

• Gene target
  Swine   heavy   light   chains   conjugated   with  
• Short name
  Swine anti IgG, heavy light chains, conjugated with
• Technique
  anti human, light, anti, IgG, antibody to, anti human antibodies, antibody Conjugates, IgGs
• Host
  Swine
• Isotype
  IgG, IgG
• Label
  Biotin
• Species
  Human, Humans
• Alternative name
  swine antibody to H. sapiens Immunoglobulin G, heavy and light chains, coupled including biotinilated
• Alternative technique
  antibodies, immunoglobulins

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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