Sucrose Phosphorylase (Biotin)

Products_type

Antibody
Products_short_name

[Sucrose Phosphorylase]
Products_name_syn

[Anti -Sucrose Phosphorylase (Biotin)]
Other_names

[sucrose phosphorylase; sucrose phosphorylase]
Other_gene_names

[RB12343]
Clonality

Polyclonal
Reactivity

Goat
Specificity

Assay by IEP resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Sucrose Phosphorylase (E. coli).
Purity

Highly Purified Purified by delipidation, salt fractionation and ion exchange chromatography.
Form

Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide, 40% glycerol. Labeled with Biotin.
Storage_stability

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Tested_application

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Gel Shift Assay (GS/EMSA)
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated