Soybean Agglutinin (SBA, Glycine max) (FITC)

Other size

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Properties

This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
Conjugation

Anti-FITC Antibody

Gene target

Soybean Agglutinin SBA Glycine max
Short name

Soybean Agglutinin (SBA, Glycine max) (FITC)
Technique

FITC, Fluorescein
Label

FITC
Alternative name

Soybean Agglutinin (SBA, Glycine v-myc myelocytomatosis viral oncogene homolog (avian) associated factor X) (fluorecein)
Alternative technique

fluorescine
Alternative to gene target

MYC associated factor X, bHLHd4, MAX and IDBG-9524 and ENSG00000125952 and 4149, protein dimerization activity, nuclei, Max and IDBG-151490 and ENSMUSG00000059436 and 17187, MAX and IDBG-646780 and ENSBTAG00000017994 and 540616

Name

Cytophotometry
Concept

Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
Qualifiers

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