Sm (Smith) Antigen, Rabbit Thymus

Products_type

Antigen
Products_short_name

[Sm (Smith) Antigen, Rabbit Thymus]
Purity

Purified from rabbit thymus by a combination of protein fractionation and column chromatography. Most contaminating antigens have been removed by immunoadsorbent methodology.
Form

Supplied as a lyophilized powder in PBS, pH 7.2, 0.02% sodium azide. Reconstitute with 100ul dH2O.
Concentration

~10 mg/mL (after reconstitution)
Storage_stability

Lyophilized and reconstituted products are stable for 6 months after receipt at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Tested_application

ELISA (EL/EIA), Western Blot (WB)
Description

Antigens are peptides or recombinant or native dependent on the production method.
About

Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name

Oryctolagus cuniculus

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data