Rituximab (FITC)

  • Catalog number
    MBS608561
  • Price
    Please ask
  • Size
    0.1 mg
  • Products_type
    Antibody
  • Products_short_name
    [Rituximab]
  • Products_name_syn
    [Anti -Rituximab (FITC)]
  • Clonality
    Monoclonal
  • Clone
    [10F381]
  • Specificity
    Recognizes the idiotypic determinants expressed by the Rituximab humanized monoclonal antibody. Does not recognize other CD20 antibodies.
  • Purity
    Affinity Purified Purified by Protein G affinity chromatography.
  • Form
    Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
  • Storage_stability
    May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
  • Tested_application
    Flow Cytometry (FC/FACS)
  • Properties
    This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • Gene target
  • Short name
    Rituximab (FITC)
  • Technique
    FITC, Fluorescein
  • Host
    Rat
  • Isotype
    IgG2a
  • Label
    FITC
  • Alternative name
    Rituximab (fluorecein)
  • Alternative technique
    fluorescine
MeSH Data
  • Name
  • Concept
    Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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