Recombinant Mouse Hyaluronidase PH-20 (Spam1) , partial

Products_type

Recombinant Protein
Products_short_name

[Hyaluronidase PH-20 (Spam1)]
Other_names

[hyaluronidase PH-20; Hyaluronidase PH-20; hyaluronidase PH-20; sperm adhesion molecule 1; Hyaluronoglucosaminidase PH-20; Sperm adhesion molecule 1; Sperm surface protein PH-20]
Products_gene_name

[Spam1]
Other_gene_names

[Spam1; Spam1; Ph-20; AV039194; Ph20; Spam; Hyal-PH20]
Purity

>85% (SDS-PAGE)
Form

Lyophilized or liquid (Format to be determined during the manufacturing process)
Storage_stability

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name

Mus musculus
Source

Recombinants or rec. proteins
Group

recombinants

Gene target

Hyaluronidase PH-20 Spam1 partial
Gene symbol

SPAM1, PAH, PIRC103, SNORD114-20, SNORD116-20, IGLVI-20, PIRC104, IGHV3-20
Short name

Recombinant Mouse Hyaluronidase PH-20 (Spam1) , partial
Technique

Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Host

E Coli or Yeast or Baculovirus or Mammalian Cell
Species

Mouse, Mouses
Alternative name

Rec. Mouse Hyaluronidase PH-20 (sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)) , partial
Alternative technique

rec, murine
Alternative to gene target

sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding), HEL-S-96n and HYA1 and HYAL1 and HYAL3 and HYAL5 and PH-20 and PH20 and SPAG15, SPAM1 and IDBG-39033 and ENSG00000106304 and 6677, hyalurononglucosaminidase activity, Plasma membranes

Identity

HGNC:11217
Gene

SPAM1
Long gene name

sperm adhesion molecule 1
Synonyms gene name
- sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
Synonyms
- HYAL5
- PH-20
- SPAG15
Synonyms name
- PH-20 hyaluronidase
GenBank acession
- L13781
Locus

7q31
Discovery year

1995-07-18
Pubmed identfication
- 8282124
- 8575780
Classification
- Hyaluronidases
VEGA ID

OTTHUMG00000157284

Identity

HGNC:5934
Gene

IGLVI-20
Long gene name

immunoglobulin lambda variable (I)-20 (pseudogene)
Synonyms gene name
- immunoglobulin lambda variable (I)-20
- immunoglobulin lambda variable (I)-20 pseudogene
Synonyms
- IGLV(I)-20
GenBank acession
- D87007
Locus

22q11.22
Discovery year

2000-05-08
Entrez gene record

28769
RefSeq identity
- NG_000002
Classification
- Immunoglobulin lambda locus at 22q11.2
VEGA ID

OTTHUMG00000151242

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data