Recombinant Methanocaldococcus jannaschii Reverse gyrase (rgy), partial
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Catalog number
MBS1283262
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Price
Please ask
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Size
Inquire
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Other size
please contact us to order other different size
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Source
Recombinants or rec. proteins
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Group
recombinants
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Description
Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
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Goup
reverse transcription
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Gene target
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Short name
Recombinant Methanocaldococcus jannaschii Reverse gyrase (rgy), partial
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Technique
Recombinant, reverse, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative name
Rec. Methanocaldococcus jannaschii Reverse gyrase (rgy), partial
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Alternative technique
rec, transctription
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MeSH Data
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Name
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Concept
Scope note:
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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