Recombinant Human Lipoprotein-Associated Phospholipase A2 (Lp-PLA2)

Products_type

Recombinant Protein
Products_short_name

[Lipoprotein-Associated Phospholipase A2 (Lp-PLA2)]
Products_name_syn

[Lp-PLA2, Recombinant]
Products_gene_name

[Lp-PLA2]
Purity

> 75% (SDS-PAGE)Affinity Chromatography with additional chromatographic steps.
Form

Lyophilized from 25 mM Na-phosphate, 0.3 M NaCl, pH 7,5 and 100 mM D-mannitol, 0.6% CHAPS.
Storage_stability

Store at -20 degree C (-15 to -30 degree C allowed).
Tested_application

Calibrator, Standard in Immunoassays
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Source

Recombinants or rec. proteins
Group

recombinants

Gene target

Lipoprotein-Associated Phospholipase Lp-PLA2
Gene symbol

PAFAH2, PLA2G1B, PLA2G7, PLA2R1
Short name

Recombinant Lipoprotein-Associated Phospholipase A2 (Lp-PLA2)
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Host

Mammalian Cell Line
Species

Human, Humans
Alternative name

Rec. H. sapiens Lipoprotein-Associated Phospholipase A2 (Lp-PLA2)
Alternative technique

rec

Identity

HGNC:8579
Gene

PAFAH2
Long gene name

platelet activating factor acetylhydrolase 2
Synonyms gene name
- platelet-activating factor acetylhydrolase 2 (40kD)
- platelet-activating factor acetylhydrolase 2, 40kDa
Synonyms
- HSD-PLA2
GenBank acession
- D87845
Locus

1p36.11
Discovery year

1998-04-03
Entrez gene record

5051
Pubmed identfication
- 8955149
- 9494101
RefSeq identity
- NM_000437
VEGA ID

OTTHUMG00000007437

Identity

HGNC:9040
Gene

PLA2G7
Long gene name

phospholipase A2 group VII
Synonyms gene name
- phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
Synonyms
- PAFAH
- LDL-PLA2
GenBank acession
- U20157
Locus

6p12.3
Discovery year

1999-09-20
Entrez gene record

7941
Pubmed identfication
- 7700381
- 8624782
Classification
- Phospholipases
VEGA ID

OTTHUMG00000014789

Identity

HGNC:9042
Gene

PLA2R1
Long gene name

phospholipase A2 receptor 1
Synonyms gene name
- phospholipase A2 receptor 1, 180kD
- phospholipase A2 receptor 1, 180kDa
Synonyms
- PLA2G1R
- PLA2IR
- PLA2-R
- CLEC13C
GenBank acession
- U17033
Locus

2q23-q24
Discovery year

1999-10-05
Pubmed identfication
- 7721806
- 7925459
Classification
- C-type lectin domain containing
VEGA ID

OTTHUMG00000154087

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data