Real-time PCR detection kit for Porcine Reproductive and Respiratory Syndrome Virus[Porcine Reproductive and Respiratory Syndrome Virus]
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Catalog numberMBS486056
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Price:
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Size150 Reactions (Advanced Version)
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Products_typePCR Kit
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DescriptionThe detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol. Real time PCR kits are used with DNA extraction kits supplied in 50 or 100 tests with polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR quantitatively (Quantitative real-time PCR), semi-quantitatively. RNA is often converted to cDNA. Often used as diagnostic tool. Ct values will have to be set for your probes.
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GroupPCR, polymerase chain reaction
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AboutTAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield. Real-time PCR amplification mixes are available for quantification with specific selected primers and with optimized buffers.
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PropertiesThermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Gene target
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Short nameReal-time PCR kit for Porcine Reproductive Respiratory Syndrome Virus[Porcine Reproductive Respiratory Syndrome Virus]
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Techniquedetection, Real-time, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
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SpeciesVirus, Viruses
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Alternative nameReal-time PCR test kit quantification reagent to measure swine Reproductive and Respiratory Syndrome Virus[swine Reproductive and Respiratory Syndrome Virus]
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Alternative techniquertpcr, kits, dna-amplification
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Virusrespiratory
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Diseasesyndrome
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MeSH Data
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Name
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ConceptScope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
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Tree numbers
- E05.393.620.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data