Rat Monoclonal [clone YL1/2] (IgG2a) to Yeast Alpha Tubulin
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Catalog number
MBS246406
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Price
730.88 USD
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Size
0.05 mg
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Products_type
Antibody
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Products_short_name
[Alpha Tubulin]
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Products_name_syn
[Anti-Alpha Tubulin Antibody (C-Terminus, clone YL1/2) IHC-plus; Alpha Tubulin; Yeast Alpha Tubulin]
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Clonality
Monoclonal
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Clone
[YL1/2]
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Reactivity
Echinoderm, Avian, Human, Mammal, Yeast
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Specificity
Recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin. This product is routinely tested in ELISA on Tubulin
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Purity
Affinity Purified
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Form
PBS, pH 7.4, 0.09% sodium azide.
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Concentration
1 mg/ml
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Storage_stability
Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.
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Tested_application
Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA), Radioimmunoassay (RIA)
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Description
The Monoclonal [clone YL1/2] (IgG2a) Yeast Alpha Tubulin is a α- or alpha protein sometimes glycoprotein present in blood.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Gene target
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Gene symbol
VPS72
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Short name
Monoclonal [clone YL1/2] (IgG2a) Yeast Alpha Tubulin
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Technique
Monoclonals or monoclonal antibodies
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Host
Rat, Ascomycota
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Isotype
IgG2a, IgG2a
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Species
Yeast, Rats, Yeasts
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Alternative name
Rat monoclonal [clonality YL1/2] (IgG2a) to Yeast a Tubulin
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Clone name
YL1
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Gene info
Product images
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