RAT IgG2b NEGATIVE CONTROL: FITC

• Catalog number
  MBS224965
• Price
  Please ask
• Size
  5x0.1 mg

• Products_type
  Isotype Control
• Products_short_name
  [IgG2b]
• Other_names
  [IgG2b, partial]
• Products_gene_name
  [IgG2b]
• Reactivity
  Negative Control
• Specificity
  RAT IgG2b NEGATIVE CONTROL
• Form
  Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration
  IgG concentration 0.1 mg/ml
• Storage_stability
  Store at +4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch
• Tested_application
  Flow Cytometry (FC/FACS)
• Description
  Isotype or positive controls by peptides, antibodies and deactivated samples. Negative control peptides or isotypes available of the different species.
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus

• Gene target
  IgG2b  
• Short name
  IgG2b NEGATIVE CONTROL: FITC
• Technique
  Negative, Control, FITC, controls, Fluorescein, negatif
• Host
  Rat
• Isotype
  IgG2b, IgG2b
• Label
  FITC
• Species
  Rat, Rats
• Alternative name
  RAT IgG2b NEGATIVE reference: fluorecein
• Alternative technique
  controls, fluorescine
• Tissue
  control

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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