RAT IgG2a NEGATIVE CONTROL: Biotin
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Catalog numberMBS224969
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Price:
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Size5x0.1 mg
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Products_typeIsotype Control
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Products_short_name[IgG2a]
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Products_gene_name[IgG2a]
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ReactivityNegative Control
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SpecificityRAT IgG2a NEGATIVE CONTROL
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FormPurified IgG conjugated to Biotin - liquid
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ConcentrationIgG concentration 0.1 mg/ml
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Storage_stabilityStore at +4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
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Tested_applicationFlow Cytometry (FC/FACS)
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PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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DescriptionIsotype or positive controls by peptides, antibodies and deactivated samples. Negative control peptides or isotypes available of the different species.
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameRattus norvegicus
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Gene target
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Short nameIgG2a NEGATIVE CONTROL:
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TechniqueNegative, Control, controls, negatif
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HostRat
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IsotypeIgG2a, IgG2a
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LabelBiotin
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SpeciesRat, Rats
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Alternative nameRAT IgG2a NEGATIVE reference: biotinilated
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Alternative techniquecontrols
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Tissuecontrol
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MeSH Data
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Name
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ConceptScope note: The technique of washing tissue specimens with a concentrated solution of a heavy metal salt and letting it dry. The specimen will be covered with a very thin layer of the metal salt, being excluded in areas where an adsorbed macromolecule is present. The macromolecules allow electrons from the beam of an electron microscope to pass much more readily than the heavy metal; thus, a reversed or negative image of the molecule is created.
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Tree numbers
- E01.370.225.500.620.670.520
- E01.370.225.750.600.670.520
- E05.200.500.620.670.520
- E05.200.750.600.670.520
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data