RAT ANTI RAT CD45.1:FITC

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Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties

This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
Conjugation

Anti-FITC Antibody
About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name

Rattus norvegicus

Identity

HGNC:9666
Gene

PTPRC
Long gene name

protein tyrosine phosphatase receptor type C
Synonyms gene
- CD45
Synonyms
- LCA
- T200
- GP180
GenBank acession
- Y00062
Locus

1q31.3-q32.1
Discovery year

1986-01-01
Entrez gene record

5788
Pubmed identfication
- 2169617
RefSeq identity
- NM_002838
Classification
- Protein tyrosine phosphatases receptor type
- Fibronectin type III domain containing
- CD molecules
VEGA ID

OTTHUMG00000035702
Locus Specific Databases
- PTPRCbase: Mutation registry for CD45 deficiency
- LRG_95

Name

Cytophotometry
Concept

Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data