Rat Anti-Mouse Kappa-FITC
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Catalog numberMBS673044
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Price:
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Size0.1 mg
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Products_typeSecondary Antibody
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Products_short_name[Rat Anti-Mouse Kappa (kappa chain specific)]
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Products_name_syn[Rat Anti-Mouse Kappa - FITC; Rat Anti-Mouse Kappa (kappa chain specific)]
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ClonalityMonoclonal
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Clone[187.1]
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ReactivityMouse
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SpecificityReacts with mouse kappa light chains. Does not react with other immunoglobulin isotypes or lambda light chains
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FormFluorescein (FITC) Conjugate
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Storage_stabilityThe purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
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Tested_applicationELISA; Flow Cytometry; Immunofluorescence
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesThis MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target
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Gene symbolIFNK, CSN3, POLK
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Short nameAnti-Mouse Kappa-FITC
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Techniqueanti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
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HostRat
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IsotypeRat IgG1 kappa
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LabelFITC
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SpeciesMouse, Mouses, Rats
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Alternative nameRat Antibody toMouse Kappa-fluorecein
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Alternative techniquemurine, antibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene nameinterferon kappa
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Synonyms gene name
- interferon, kappa
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GenBank acession
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Locus
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Discovery year2004-02-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interferons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecasein kappa
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Synonyms gene
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Synonyms gene name
- casein, kappa
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GenBank acession
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Locus
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Discovery year1997-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameDNA polymerase kappa
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Synonyms gene
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Synonyms gene name
- polymerase (DNA directed) kappa
- polymerase (DNA) kappa
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-08-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- DNA polymerases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data