Rat Anti-Mouse CD86/B7-2-UNLB
-
Catalog number
MBS673451
-
Price
Please ask
-
Size
0.5 mg
-
-
Products_type
Antibody
-
Products_short_name
[CD86/B7-2]
-
Products_name_syn
[Rat Anti-Mouse CD86/B7-2 - Purified, Unlabeled Antibody; Rat Anti-Mouse CD86/B7-2]
-
Other_names
[CD86/B7-2 costimulatory molecule; CD86/B7-2 costimulatory molecule; CD86 antigen; B7-1 costimulatory molecule]
-
Other_gene_names
[LOC554318]
-
Clonality
Monoclonal
-
Clone
[GL1]
-
Reactivity
Mouse
-
Specificity
Mouse CD86, the B7-2 co-stimulatory molecule and a ligand for CD28 and CD152/CTLA-4 CD86, also known as B7-2, is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes.^1-5 CD86 expression is rapidly upregulated by B-cell specific stimuli with peak expression at 18-42 hours after stimulation.^1, 5 CD86, along with CD80/B7-1, is an important accessory molecule in T cell costimulation via its interaction with CD28 and CD152/CTLA-4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response.^1-9 The monoclonal antibody GL1 blocks mixed lymphocyte reactions and stimulation of T cells by antigen-presenting cells.^1, 3, 5
-
Form
Purified (UNLB) Antibody
-
Storage_stability
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or aminecontaining buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 7 (R-PE-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
-
Tested_application
Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus, Rattus norvegicus
-
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
-
Gene target
-
Gene symbol
CD86, CD276, HHLA2, RNY4P2, LRRC23, PCDHGB7, NCR3LG1, CD274, TLX1NB, CD80
-
Short name
Anti-Mouse CD86/B7-2-UNLB
-
Technique
anti-, Mouse, anti, antibody to, antibodies, mouses
-
Host
Rat
-
Isotype
Rat (Louvain) IgG2a kappa
-
Species
Mouse, Mouses, Rats
-
Alternative name
Rat Antibody toMouse CD86 molecule/B7-2-UNLB
-
Alternative technique
murine, antibodies
-
Alternative to gene target
CD86 molecule, B7-2 and B7.2 and B70 and CD28LG2 and LAB72, CD86 and IDBG-52336 and ENSG00000114013 and 942, coreceptor activity, Cell surfaces, Cd86 and IDBG-158071 and ENSMUSG00000022901 and 12524, CD86 and IDBG-633264 and ENSBTAG00000013118 and 414345
-
Gene info
-
Identity
-
Gene
-
Long gene name
CD86 molecule
-
Synonyms gene
-
Synonyms gene name
- CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year
1994-12-07
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- B7 family
- CD molecules
- V-set domain containing
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene name
CD276 molecule
-
Synonyms gene name
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year
2005-03-04
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- C2-set domain containing
- V-set domain containing
- B7 family
- CD molecules
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene name
HERV-H LTR-associating 2
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year
1999-09-07
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- C1-set domain containing
- V-set domain containing
- B7 family
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene name
RNY4 pseudogene 2
-
Synonyms gene name
- RNA, Y4 small cytoplasmic (associated with Ro protein) pseudogene 2
- RNA, Ro-associated Y4 pseudogene 2
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year
1993-08-25
-
Entrez gene record
-
RefSeq identity
Gene info
Gene info
Gene info
Gene info
-
Identity
-
Gene
-
Long gene name
CD274 molecule
-
Synonyms gene
-
Synonyms gene name
- programmed cell death 1 ligand 1
- CD274 antigen
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
2003-11-13
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Receptor ligands
- C2-set domain containing
- V-set domain containing
- B7 family
- CD molecules
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene name
TLX1 neighbor
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
2009-08-14
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Long non-coding RNAs with non-systematic symbols
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene name
CD80 molecule
-
Synonyms gene
-
Synonyms gene name
- CD80 antigen (CD28 antigen ligand 1, B7-1 antigen)
- CD80 molecule
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year
1993-12-14
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- B7 family
- CD molecules
- C2-set domain containing
- V-set domain containing
-
VEGA ID
Product images
Similar products
