Rat Anti-Mouse CD45/LCA-LE/AF

• Catalog number
  MBS673374
• Price
  Please ask
• Size
  0.5 mg

• Products_type
  Antibody
• Products_short_name
  [CD45 (LCA)]
• Products_name_syn
  [Rat Anti-Mouse CD45/LCA - Low Endotoxin/Azide-Free; Rat Anti-Mouse CD45 (LCA)]
• Clonality
  Monoclonal
• Clone
  [I3/2.3]
• Reactivity
  Mouse
• Specificity
  Mouse CD45 (T200 or leukocyte common antigen/LCA, all isoforms) CD45 is the common leukocyte antigen and is expressed on all cells of hematopoietic origin except erythrocytes.^1-4 CD45 exists as a number of different isoforms which result from alternative RNA splicing of exons 4, 5 and 6. The I3/2.3 monoclonal antibody recognizes a framework epitope present on all CD45 isoforms.^1-4 The CD45 molecule has been implicated as playing a key role in thymic development and in antigen-driven proliferation of T cells.^4
• Form
  Low Endotoxin, Azide - Free (LE/AF)
• Storage_stability
  The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The Spectral Red (SPRD), R-phycoerythrin-Texas Red (R-PE-TXRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5), and allophycocyanin-Cyanine 7 (APCCY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 degree C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree The Alexa Fluor 488 (AF488) conjugate is supplied as 0.1mg in 0.2mL of PBS/NaN3. Store at 2-8 degree C. All products should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label if stored as directed.
• Tested_application
  Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

• Gene target
  CD45/LCA-LE/AF  
• Gene symbol
  PTPRC, CLTA, FUT3, HPS4, PTPRCAP, NPAS4, GUCY2D
• Short name
  Anti-Mouse CD45/LCA-LE/AF
• Technique
  anti-, Mouse, anti, antibody to, antibodies, mouses
• Host
  Rat
• Isotype
  Rat IgG2b
• Species
  Mouse, Mouses, Rats
• Alternative name
  Rat Antibody toMouse CD45/LCA-LE/AF
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:9666
• Gene
  PTPRC
• Long gene name
  protein tyrosine phosphatase receptor type C
• Synonyms gene
  • CD45
• Synonyms
  • LCA
  • T200
  • GP180
• GenBank acession
  • Y00062
• Locus
  1q31.3-q32.1
• Discovery year
  1986-01-01
• Entrez gene record
  5788
• Pubmed identfication
  • 2169617
• RefSeq identity
  • NM_002838
• Classification
  • Protein tyrosine phosphatases receptor type
  • Fibronectin type III domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000035702
• Locus Specific Databases
  • PTPRCbase: Mutation registry for CD45 deficiency
  • LRG_95

Gene info

• Identity
  HGNC:2090
• Gene
  CLTA
• Long gene name
  clathrin light chain A
• Synonyms gene name
  • clathrin, light polypeptide (Lca)
  • clathrin, light chain A
• Synonyms
  • Lca
• Locus
  9p13.3
• Discovery year
  1991-08-08
• Entrez gene record
  1211
• Pubmed identfication
  • 7713494
• RefSeq identity
  • NM_007096
• Classification
  • Clathrin subunits
• VEGA ID
  OTTHUMG00000019896

Gene info

• Identity
  HGNC:4014
• Gene
  FUT3
• Long gene name
  fucosyltransferase 3 (Lewis blood group)
• Synonyms gene
  • LE
• Synonyms gene name
  • fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group included)
• Synonyms
  • CD174
• Synonyms name
  • galactoside 3(4)-L-fucosyltransferase
• Locus
  19p13.3
• Discovery year
  1993-04-08
• Entrez gene record
  2525
• Pubmed identfication
  • 1977660
  • 1740457
• RefSeq identity
  • NM_000149
• Classification
  • Fucosyltransferases
  • Blood group antigens
  • CD molecules
• VEGA ID
  OTTHUMG00000180614
• Locus Specific Databases
  • Blood Group Antigen Mutation Database
  • LRG_800

Gene info

• Identity
  HGNC:15844
• Gene
  HPS4
• Long gene name
  HPS4 biogenesis of lysosomal organelles complex 3 subunit 2
• Synonyms gene name
  • Hermansky-Pudlak syndrome 4
• Synonyms
  • KIAA1667
  • LE
  • BLOC3S2
• Locus
  22q12.1
• Discovery year
  2001-06-28
• Entrez gene record
  89781
• Pubmed identfication
  • 11836498
  • 12663659
• RefSeq identity
  • NM_022081
• Classification
  • Biogenesis of lysosomal organelles complex 3
• VEGA ID
  OTTHUMG00000030459
• Locus Specific Databases
  • Mutations of the Human light ear Gene (le, HPS4)
  • LRG_590

Gene info

• Identity
  HGNC:9667
• Gene
  PTPRCAP
• Long gene name
  protein tyrosine phosphatase receptor type C associated protein
• Synonyms
  • LPAP
  • CD45-AP
• Locus
  11q13.2
• Discovery year
  1996-04-22
• Entrez gene record
  5790
• RefSeq identity
  • NM_005608
• VEGA ID
  OTTHUMG00000150325

Gene info

• Identity
  HGNC:18983
• Gene
  NPAS4
• Long gene name
  neuronal PAS domain protein 4
• Synonyms
  • PASD10
  • NXF
  • Le-PAS
  • bHLHe79
• GenBank acession
  • AB049469
• Locus
  11q13.2
• Discovery year
  2005-09-26
• Entrez gene record
  266743
• Pubmed identfication
  • 14701734
• RefSeq identity
  • NM_178864
• Classification
  • Basic helix-loop-helix proteins
• VEGA ID
  OTTHUMG00000167045

Gene info

• Identity
  HGNC:4689
• Gene
  GUCY2D
• Long gene name
  guanylate cyclase 2D, retinal
• Synonyms gene
  • CORD6
  • LCA
  • GUC2D
  • GUC1A4
• Synonyms gene name
  • cone rod dystrophy 6
  • guanylate cyclase 2D, membrane (retina-specific)
• Synonyms
  • retGC
  • RETGC-1
  • ROS-GC1
  • CYGD
  • LCA1
• Synonyms name
  • rod outer segment membrane guanylate cyclase
  • retinal guanylate cyclase 1
• GenBank acession
  • L26921
• Locus
  17p13.1
• Discovery year
  1993-11-09
• Entrez gene record
  3000
• Pubmed identfication
  • 1356371
  • 12552567
• RefSeq identity
  • NM_000180
• Classification
  • Guanylate cyclase receptors
• VEGA ID
  OTTHUMG00000108169
• Locus Specific Databases
  • Mutations of the Retinal Guanylate Cyclase Gene and the Retinal Guanylate Cyclase-activating Protein Gene

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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