Rat Anti-Mouse CD3e-LE/AF
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Catalog numberMBS673153
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Price:Ask for price
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Size0.5 mg
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Products_typeAntibody
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Products_short_name[CD3 epsilon]
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Products_name_syn[Rat Anti-Mouse CD3e - Low Endotoxin/Azide-Free; Rat Anti-Mouse CD3 epsilon]
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Other_names[CD3 epsilon; CD3 epsilon]
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Other_gene_names[cd3e]
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ClonalityMonoclonal
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Clone[C363.29B (YCD3-1)]
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ReactivityMouse
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SpecificityMouse CD3 epsilon (Mr 25 kD) CD3 epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains.^1, 2 CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. The monoclonal antibody C363.29B recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.^1 In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naïve T cell in vitro. Immobilized C363.29 monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present.^1, 3, 4 The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody.^1
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FormLow Endotoxin, Azide - Free (LE/AF)
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Storage_stabilityThe purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
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Tested_applicationFlow Cytometry; Immunohistochemistry; Immunoprecipitation
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target
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Gene symbolFUT3, HPS4, NPAS4
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Short nameAnti-Mouse CD3e-LE/AF
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Techniqueanti-, Mouse, anti, antibody to, antibodies, mouses
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HostRat
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IsotypeRat IgG2b kappa
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SpeciesMouse, Mouses, Rats
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Alternative nameRat Antibody toMouse CD3e molecule, epsilon (CD3-TCR complex)-LE/AF
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Alternative techniquemurine, antibodies
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Alternative to gene targetCD3e molecule, epsilon (CD3-TCR complex), IMD18 and T3E and TCRE, CD3E and IDBG-73617 and ENSG00000198851 and 916, protein heterodimerization activity, Cell surfaces, Cd3e and IDBG-159085 and ENSMUSG00000032093 and 12501, CD3E and IDBG-631260 and ENSBTAG00000015710 and 281054
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Gene info
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Identity
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Gene
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Long gene namefucosyltransferase 3 (Lewis blood group)
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Synonyms gene
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Synonyms gene name
- fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group included)
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Synonyms
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Synonyms name
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Locus
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Discovery year1993-04-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Fucosyltransferases
- Blood group antigens
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameHPS4 biogenesis of lysosomal organelles complex 3 subunit 2
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Synonyms gene name
- Hermansky-Pudlak syndrome 4
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Synonyms
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Locus
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Discovery year2001-06-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Biogenesis of lysosomal organelles complex 3
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameneuronal PAS domain protein 4
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Basic helix-loop-helix proteins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
