Rat Anti-Mouse CD24/HSA-FITC
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Catalog numberMBS673591
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Price:
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Size5x0.5 mg
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Products_typeAntibody
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Products_short_name[CD24/HSA]
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Products_name_syn[Rat Anti-Mouse CD24/HSA - FITC; Rat Anti-Mouse CD24/HSA]
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ClonalityMonoclonal
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Clone[30-F1]
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ReactivityMouse
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SpecificityMouse CD24/heat stable antigen (HSA) CD24 is expressed in B cells until their terminal differentiation into plasma cells.1 It has been used widely as a marker to fractionate different B-lineage differentiation stages.10 CD24 has been found on a variety of other hematopoietic and neural cell types, and is now classified as a costimulatory molecule.2-4 The CD24/HSA molecule is composed mostly of carbohydrate, with a protein core of only 31-35 amino acids in the human and 27 amino acids in the mouse.1, 3 The molecular mass is variable, ranging from 38-70 kDa.3 CD24 is now included in the large group of phosphatidylinositol-anchored membrane proteins.1-4 Studies with purified CD24 applied to latex beads have demonstrated a self-binding property, suggesting that the molecule is capable of promoting homotypic aggregation, binding and cell adhesion.3 This is supported by earlier observations of the blockade of B cell lymphoblast aggregation with anti- CD24 monoclonal antibody.6 Recent studies of murine CD24 have assigned a role for CD24/HSA as a ligand for P-selectin, a calcium-dependent lectin.7
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FormFluorescein (FITC) Conjugate
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Storage_stabilityThe purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
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Tested_applicationFlow Cytometry; Immunoprecipitation
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesThis MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target
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Gene symbolCD24, MIR3591, MIR3150A, MIR146A, MIR374A, MIR18A, MIR301A, MIR148A, MIR323B, MIR193A
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Short nameAnti-Mouse CD24/HSA-FITC
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Techniqueanti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
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HostRat
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IsotypeRat IgG2c kappa
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LabelFITC
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SpeciesMouse, Mouses, Rats
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Alternative nameRat Antibody toMouse CD24/HSA-fluorecein
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Alternative techniquemurine, antibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene nameCD24 molecule
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Synonyms gene name
- CD24 antigen (small cell lung carcinoma cluster 4 antigen)
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Synonyms
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Locus
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Discovery year1994-09-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 3591
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Synonyms
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Locus
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Discovery year2011-05-02
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNAs
Gene info
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Identity
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Gene
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Long gene namemicroRNA 3150a
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Synonyms gene
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Synonyms gene name
- microRNA 3150
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Synonyms
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Locus
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Discovery year2010-04-30
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNAs
Gene info
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Identity
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Gene
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Long gene namemicroRNA 146a
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Synonyms gene
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Synonyms gene name
- microRNA 146
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR146 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 374a
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Synonyms gene
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Synonyms gene name
- microRNA 374
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Synonyms
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Locus
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Discovery year2004-07-22
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR374 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 18a
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Synonyms gene
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Synonyms gene name
- microRNA 18
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR17 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 301a
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Synonyms gene
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Synonyms gene name
- microRNA 301
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR130 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 148a
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Synonyms gene
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Synonyms gene name
- microRNA 148
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR148/152 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 323b
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Synonyms gene
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Synonyms gene name
- microRNA 453
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Synonyms
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Locus
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Discovery year2010-04-30
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR154 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 193a
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Synonyms gene
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Synonyms gene name
- microRNA 193
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR193 family
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data