-
Products_type
Antibody
-
Products_short_name
[CD206]
-
Other_names
[macrophage mannose receptor 1; Macrophage mannose receptor 1; macrophage mannose receptor 1; MMR; mannose receptor, C type 1; CD_antigen: CD206]
-
Products_gene_name
[CD206]
-
Other_gene_names
[Mrc1; Mrc1; MR; CD206; AW259686; MMR]
-
Clonality
Monoclonal
-
Clone
[MR5D3]
-
Reactivity
Mouse
-
Specificity
Rat anti mouse CD206 antibody, clone MR5D3 recognizes the mouse mannose receptor, a ~175 kDa type 1 membrane glycoprotein that is also known as CD206. CD206 is expressed on most tissue macrophages, certain endothelial cells and in vitro derived dendritic cells (Zamze et al. 2002). The mannose receptor, CD206, is composed of a N-terminal cysteine-rich domain, a fibronectin type II domain, eight tandemly arranged C-type lectin domains (CTLD), a transmembrane domain, and a cytoplasmic domain. The terminal cysteine-rich domain binds sulfated sugars, and the CTLD recognizes carbohydrates terminating in mannose, fucose and N-acetylglucosamine, all sugars found on microorganisms and on some endogenous proteins (Su et al. 2005). Rat anti mouse CD206 antibody, clone MR5D3 has been reported to be non-inhibitory for the binding of the mannose receptor to carbohydrate ligands (Zamze et al. 2002). Clone MR5D3 has also been shown to work in Western Blot (Martinez-Pomares et al. 2003 and Su et al. 2005).
-
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
-
Form
Purified IgG - liquid
-
Storage_stability
Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
-
Tested_application
Flow Cytometry (FC/FACS)*, Immunohistochemistry - Frozen (IHC-F), Immunoprecipitation (IP), Immunofluorescence (IF) *CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation.
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus, Rattus norvegicus
-
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
