RAT ANTI DOG MHC CLASS II MONOMORPHIC:FITC

• Catalog number
  MBS214761
• Price
  Please ask
• Size
  100 Tests

• Products_type
  Antibody
• Products_short_name
  [MHC CLASS II MONOMORPHIC]
• Clonality
  Monoclonal
• Clone
  [YKIX334.2]
• Form
  FITC Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration
  IgG concentration 0.1 mg/ml
• Storage_stability
  Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
• Tested_application
  Flow cytometry (FC/FACS)
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  Canine or Canis Lupus is mostly Beagle used for drug research. MyBioSource produces ELISA test kits and polyclonal antibodies. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus

• Gene target
  MHC   CLASS   MONOMORPHIC  
• Short name
  ANTI MHC CLASS II MONOMORPHIC:FITC
• Technique
  anti, FITC, antibody to, Fluorescein
• Host
  Rat
• Isotype
  IgG2a
• Label
  FITC
• Species
  Dog, Dogs, Rats
• Alternative name
  RAT antibody to canine Major histocompabitility complex CLASS II MONOMORPHIC:fluorecein
• Alternative technique
  antibodies, fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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