Rabbit Polyclonal (IgG) to Sheep SFN / Stratifin / 14-3-3 Sigma
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Catalog numberMBS244926
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PricePlease ask
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Size0.05 mL
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Products_typeAntibody
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Products_short_name[SFN / Stratifin / 14-3-3 Sigma]
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Products_name_syn[Anti-SFN / Stratifin / 14-3-3 Sigma Antibody (N-Terminus) IHC-plus; SFN; 14-3-3 sigma; 14-3-3 protein sigma; HME1; Stratifin; YWHAS; Sheep SFN; Stratifin; 14-3-3 Sigma]
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Other_names[14-3-3 protein sigma; 14-3-3 protein sigma; 14-3-3 protein sigma; 14-3-3 sigma; epithelial cell marker protein 1; stratifin; Epithelial cell marker protein 1; Stratifin]
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Products_gene_name[SFN]
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Other_gene_names[SFN; SFN; YWHAS; HME1]
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ClonalityPolyclonal
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ReactivityBovine, Chicken, Human, Mouse, Rabbit, Rat, Sheep Predicted Reactivity: Mammal (at least 90% immunogen sequence identity)
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SpecificityRecognizes 14-3-3 sigma in all mammals. It is a member of the 14-3-3 family which consists of 30 kD proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 sigma is expressed mostly in epithelial cells and has been associated with cancers including breast, nasopharyngeal, endometrial adenocarcinoma, prostate, epithelial ovarian, colorectal, adenoid cystic, and papillary. 14-3-3 sigma binds c-fos activating the MAPK pathway. Sequestration of Bax by 14-3-3 sigma inhibits apoptosis whereas sequestration of 14-3-3 sigma by CARP-1 induces it. Mitosis is regulated by 14-3-3 sigma through binding keratin filaments and sequestering cyclin B1 and cdc2. p73-dependent induction of 14-3-3 sigma regulates chemo sensitivity in breast cancers with p53 mutations. In reaction to DNA damage and along with RACK1, 14-3-3 sigma targets, DeltaNp63alpha for proteasome degradation. 14-3-3 also negatively regulates both the glucocorticoid receptor and the activity of PKB/Akt which stabilizes p27 Kip1. May not react with recombinant proteins that are not N-acetylated.
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FormSerum, 0.09% sodium azide.
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Storage_stabilityLong term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
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Tested_applicationImmunohistochemistry (IHC - Paraffin), Western Blot (WB)
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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GroupPolyclonals and antibodies
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolYWHAQ, YWHAG, YWHAZ, MIR1302-3, MIR509-3, MIR7-3, PIRC13, PIRC11
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Short nameRabbit Polyclonal (IgG) SFN / Stratifin / 14-3-3 Sigma
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TechniquePolyclonal, Rabbit, IgG, IgGs, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeIgG, IgG
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SpeciesSheep, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
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Alternative nameproduction species: rabbit polyclonal (Immunoglobulin G) to ovine stratifin / Stratifin / 14-3-3 Sigma
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Alternative techniquepolyclonals, rabbit-anti, immunoglobulins
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Alternative to gene targetstratifin, YWHAS, SFN and IDBG-94738 and ENSG00000175793 and 2810, phosphoprotein binding, nuclei, Sfn and IDBG-195282 and ENSMUSG00000047281 and 55948, SFN and IDBG-647781 and ENSBTAG00000009223 and 528453
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Gene info
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Identity
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Gene
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Long gene nametyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta
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Synonyms gene name
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
- protein, theta
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-08-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- 14-3-3 phospho-serine/phospho-threonine binding proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma
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Synonyms gene name
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-09-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- 14-3-3 phospho-serine/phospho-threonine binding proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
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Synonyms gene
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Synonyms gene name
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-09-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- 14-3-3 phospho-serine/phospho-threonine binding proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1302-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1302 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 509-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year2007-10-24
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR506 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 7-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR7 family
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 13
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 11
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
