Rabbit anti Porcine Malic dehydrogenase

• Catalog number
  MBS573145
• Price
  Please ask
• Size
  5x10 mg

• Products_type
  Antibody
• Products_short_name
  [Malic dehydrogenase]
• Other_names
  [Malic dehydrogenase; Malate dehydrogenase; malate dehydrogenase]
• Other_gene_names
  [mdh; mdh]
• Clonality
  Polyclonal
• Reactivity
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Specificity
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
• Purity
  The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
• Form
  Purified hyperimmune rabbit IgG Iyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
• Concentration
  10mg/ml
• Storage_stability
  Handling: The Iyophilised IgG fraction is shipped at ambient temperature and may be stored at +4 Degree C; prolonged storage at or below -20 Degree C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 Degree C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 Degree C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Storage & Shelf -life: Lyophilised at +4 Degree C = at least 10 years Reconstituted at or below -20 Degree C = 3-5 years Reconstituted at +4 Degree C =7 days
• Tested_application
  Indirect Immunofluorescence (IF), ELISA (EIA), Dot Blot (DB), Immunoblotting (IB)
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• About
  Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus

• Gene target
  Malic   dehydrogenase  
• Gene symbol
  ME1, ME2, ME3
• Short name
  Rabbit anti Porcine Malic dehydrogenase
• Technique
  Rabbit, anti, antibody to
• Host
  Rabbit, Rabbits
• Species
  Porcine
• Alternative name
  production species: rabbit antibody to swine Malic dehydrogenase
• Alternative technique
  rabbit-anti, antibodies

Gene info

• Identity
  HGNC:6983
• Gene
  ME1
• Long gene name
  malic enzyme 1
• Synonyms gene name
  • malic enzyme 1, NADP(+)-dependent, cytosolic
• Synonyms name
  • malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+))
  • NADP-dependent malic enzyme
• GenBank acession
  • X77244
• Locus
  6q14.2
• Discovery year
  2001-06-22
• Entrez gene record
  4199
• Pubmed identfication
  • 8187880
• VEGA ID
  OTTHUMG00000015111

Gene info

• Identity
  HGNC:6984
• Gene
  ME2
• Long gene name
  malic enzyme 2
• Synonyms gene name
  • malic enzyme 2, NAD(+)-dependent, mitochondrial
• Synonyms name
  • NAD-dependent malic enzyme, mitochondrial
  • malate dehydrogenase (oxaloacetate-decarboxylating)
• GenBank acession
  • M55905
• Locus
  18q21.2
• Discovery year
  2001-06-22
• Entrez gene record
  4200
• Pubmed identfication
  • 1993674
• RefSeq identity
  • NM_002396
• VEGA ID
  OTTHUMG00000132694

Gene info

• Identity
  HGNC:6985
• Gene
  ME3
• Long gene name
  malic enzyme 3
• Synonyms gene name
  • malic enzyme 3, NADP(+)-dependent, mitochondrial
• Synonyms name
  • malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+))
• GenBank acession
  • X79440
• Locus
  11q14.2
• Discovery year
  2000-01-12
• Entrez gene record
  10873
• Pubmed identfication
  • 7818469
• VEGA ID
  OTTHUMG00000167217

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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