Rabbit anti Bee venom Phospholipase A2, conjugated with Biotin
#
-
Catalog numberMBS573423
-
Price:
-
Size5x1 mL
-
-
Products_typeAntibody
-
Products_short_name[Bee venom Phospholipase A2]
-
ClonalityPolyclonal
-
ReactivityCross-reactivities against enzymes of other sources may occur but have not been determined.
-
SpecificityBiotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to phospholipase A2 from bee venom.
-
FormBiotin; Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
-
Storage_stabilityThe lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product
-
Tested_applicationELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), Dot Blot (DB), Immunoblotting (IB)
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
-
ConjugationBiotinylated
-
AboutRabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin nameOryctolagus cuniculus
-
Gene target
-
Short nameRabbit anti Bee venom Phospholipase A2, conjugated with
-
TechniqueRabbit, anti, antibody to, antibody Conjugates
-
HostRabbit, Rabbits
-
LabelBiotin
-
Alternative nameproduction species: rabbit antibody to Bee venom Phospholipase A2, coupled including biotinilated
-
Alternative techniquerabbit-anti, antibodies
-
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data