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Products_type
Antibody
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Products_short_name
[Bacillus cereus Phospholipase C]
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Clonality
Polyclonal
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Reactivity
Cross-reactivities against enzymes of other sources may occur but have not been determined.
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Specificity
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition.
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Purity
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography
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Form
Biotin-coupled hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). 1 vial with 1ml immunoconjugate, lyophilized from PBS
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Storage_stability
The lyophilized conjugate is shipped at ambient temperature and may be stored at 4 degree C; prolonged storage at or below -20 degree C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below - 20 degree C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4 degree C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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Tested_application
ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), Dot Blot (DB), Immunoblotting (IB)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Bacillus Anthracis and Cereus are the most known Bacilli. Anthrax has a very high toxin level and is used in Biologic warfare. However 1 is a harmless product.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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About
Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus