Phosphotyrosine, mixed clones

Products_type

Antibody
Products_short_name

[Phosphotyrosine, mixed clones]
Products_name_syn

[Anti -Phosphotyrosine, mixed clones]
Clonality

Monoclonal
Clone

[3G244]
Specificity

PY-Plus reacts specifically with tyrosine-phosphorylated proteins. It shows no crossreactivity to phosphoserine or phosphothreonine. On Western blots, specific inhibition of each individual antibody and the cocktail reactivity can be achieved by pre-incubation of the antibody with 20 mM phosphotyrosine, whereas pre-incubation with 20 mM phosphoserine or 20 mM phosphothreonine is ineffective.
Purity

Affinity Purified Purified by immunoaffinity chromatography.
Form

Supplied as a liquid in phosphate buffered saline, pH 7.4, 40% glycerol and 0.1% proclin.
Storage_stability

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Tested_application

Western Blot (WB), Immunoprecipitation (IP)