Peroxidase Conjugated Affinity Purified anti-Mouse IgG [H&L] [Donkey]
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Catalog numberMBS417959
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Price:
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Size0.25 mg
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Products_typeSecondary Antibody
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Products_short_name[IgG [H&L]]
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Products_gene_name[IgG]
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ReactivityMouse
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Concentration1.0 mg/ml (by UV absorbance at 280 nm)
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Storage_stabilityThis product is stable for several weeks at 4 degree C as an undiluted liquid. For extended storage aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
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Tested_applicationImmunoblotting, Western Blot (WB), Dot Blot, ELISA (EIA), Immunoperoxidase Electron Microscopy, Immunohistochemistry (IHC), Other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency
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TestA high affinity purification column was use to purify Conjugated anti-Mouse IgG [H L] [ ] by MyBioSource by chromatographic size exclusion. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Description1 of protein A or G purified by MyBioSource will give more specificity than crude serum. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Latin nameEquus africanus asinus, Mus musculus
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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ConjugatePeroxidases with EC number 1.11.1.x are catalyzing the reaction of ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH
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Gene target
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Short nameConjugated anti-Mouse IgG [H L] [ ]
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Techniqueaffinity, purified, anti-, Mouse, anti, IgG, antibody to, antibodies, antibody Conjugates, IgGs, mouses, purifications
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HostDonkey, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
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IsotypeIgG, Heavy & Light chains, IgG
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Labelperoxidase
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SpeciesMouse, Donkeys, Mouses
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Alternative namePeroxidase coupled protein enriched Antibody toMouse Immunoglobulin G [heavy + light chain] [Donkey]
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Alternative techniquepure, murine, antibodies, immunoglobulins
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data